Alnylam Pharmaceuticals, Inc.

With me today are Yvonne Greenstreet, Chief Executive Officer, Bova Tanguilar, Chief Commercial Officer, Krish Pagard, Chief Research and Development Officer, and Jeff Fulton, Chief Financial Officer. For those of you participating via conference call, the accompanying slides can be accessed by going to the events section of the investors page of our website, investors.onilam.com slash events. During today's call, as outlined in slide two, Yvonne will offer introductory remarks and provide some general context. Tova will provide an update on our global commercial progress. Bush will review pipeline updates and clinical progress, and Jeff will review our financials and guidance, followed by a summary of upcoming milestones before we open the call to your questions. I'd like to remind you that this call will contain remarks concerning Alnylam's future expectations, plans, and prospects, which constitute forward-looking statements for the purposes of the safe harbor provision under the Private Securities Litigation Reform Act of 1995. Actual results may differ materially from those indicated by these forward-looking statements as a result of various important factors, including those discussed in our most recent periodic report on file of the SEC. In addition, any forward-looking statements represent our views as of the date of this recording and should not be relied upon as representing our views as of any subsequent date. We specifically disclaim any obligation to update such statements. With that, I'd like to turn the call over to Yvonne. Yvonne?

Thanks, Christine, and thank you, everyone, for joining the call today. As shown in our Q2 results announced today, our 9M is firing on all cylinders. And we're swiftly establishing ourselves as a top-tier biotech company. We're doing so by focusing on three core elements of the business that we believe will drive sustainable growth and value creation for years to come. The first is TTR leadership. As highlighted in today's press release, the launch is off to a very strong start. And whilst it's still early days, we're encouraged by the pace. And we're deeply focused on laying the groundwork for long-term leadership in TTR. The next is growth through innovation, focused on the potential multi-billion dollar opportunities within our pipeline and an R&D engine set up to deliver sustainable innovation and value creation. To that end, I'd like to acknowledge the recent promotion of Pushkol Garg to Chief Research and Development Officer. Pushkol has been instrumental in progressing our pipeline. And in this role, Pushkol will oversee an integrated R&D organization to drive this exciting pipeline and platform into the future. Congratulations, Pushkol. The third element is strong financial performance with robust commercial execution and disciplined capital allocation approach, providing us with the opportunity to sustain profitability going forward. As Tolga and Jeff will highlight later, The strong therapeutic profile of Ambutra and ATTR-CM, combined with a large and underserved market, positioned this as a flagship commercial franchise with robust and durable long-term growth potential. And of course, all of this is underpinned by a best-in-class team and our award-winning culture. Our results this quarter fit within each of these strategic pillars and represent one of the most impactful quarters to date for our nylon. Our commercial performance was driven by TTR franchise revenues of $544 million, 77% year-over-year growth, with growth largely attributable to the Amfutra CM launch. This was just the first full quarter of the ATTR CM launch, and as of June 30th, approximately 1,400 cardiomyopathy patients were receiving Amfutra, and this is a remarkable achievement. This performance reflects results from our cardiomyopathy launch in the U.S. only. International markets are coming online for Ambutra for CM and are expected to begin to contribute to the CM launch in the second half of the year. Kudos to our team for delivering these impressive early results. In addition to these commercial results, we continue to advance our leading pipeline of RNAi therapeutics. We initiated the Triton CM Phase 3 study of Nucreceram further establishing our commitment to leadership in TTR and are pleased to be announcing today that the FDA has granted fast-track designation to Nucleusiran for ATTR-CM. We also just shared encouraging phase one multi-dose data for Mifelsiran in Alzheimer's disease and kicked off a phase one study for ALN4324 in type 2 diabetes earlier in the quarter. And with regard to financial performance, we're reporting our strongest quarter to date as a company with $672 million in total net product revenues or 64% growth year over year. As a result, we've increased our total net product revenues guidance for 2025 from a range of $2.05 to $2.25 billion to a revised range of $2.65 billion to $2.8 billion representing an increase of $575 million, or 27% at the midpoint, underscoring our confidence in the ATTR-CM launch and our other commercial products in the balance of the year. So zooming out from the success of Q2, this progress represents stellar execution towards our nylon pizza fits by 25 goals, which we seek to achieve by the end of this year. Doing so will further establish our nylon, as a unique top-tier biotech company delivering sustainable innovation to patients for many years to come. With that, let me now turn the call over to Tolga for a view of our commercial performance. Tolga?

Thanks, Ivan, and good morning, everyone. I'm excited to share with you the results of our Q2 commercial performance. As Ivan indicated, we are firing on all cylinders, and our Q2 performance was exceptional. for the full portfolio. On a global basis, our commercial portfolio delivered $672 million in net product revenues, representing 64% year-over-year and 43% quarter-over-quarter growth. As you will see in a moment, the US TTR performance was the major driver of growth given the ATTR-CM launch for Ambutrem. It is also encouraging that we saw very robust double-digit growth compared with Q1 across both our TTR and Rare franchises across the globe. All parts of our business are operating with focus and excellence. Let's quickly start with our Rare franchise. Our Givlari and Oxlumo teams stayed focused and delivered $128 million in combined Q2 sales, up 24% versus last year. Growth was largely demand driven with a tailwind from favorable give large growth to net adjustments in the US. Now, turning our attention to TTR franchise where we delivered $544 million in global net product revenues during the quarter, representing a 77% increase compared with the second quarter of 24 and a robust 51% increase compared with the first quarter of 2025. In the US, combined Q2 sales of Onpatro and Ambutra rose 80%, up roughly $170 million from Q1, driven primarily by Ambutra's ATTR-CM launch. We closed the quarter with approximately 1,400 cardiomyopathy patients on therapy, contributing an estimated $150 million in revenue. This performance was fueled by strong execution and faster than anticipated access across payers and providers. Regarding the year-over-year dynamics, the US TTR franchise grew 125% compared with the second quarter of 2024, primarily driven by the significant increase in demand from the ATTR CRM launch that I just highlighted. Turning to our international markets, we delivered 18% year-over-year growth, driven by continued strength in our HATTR PM business, which remains a solid growth engine. Importantly, we have yet to recognize any ATTR CM revenue internationally, as launches in Germany and Japan are slated to begin contributing in third quarter. Now, let me provide some additional perspectives on the US TTR revenue dynamics, where the franchise achieved $383 million in the second quarter, representing a very robust 80% quarter-over-quarter growth. While we don't have the ability to report revenue by indication, the underlying trend is clear. From Q1 24 through Q1 25, the US TTR franchise delivered steady growth of around $15 to $20 million on average every quarter. In Q2 2025, we saw a pronounced step change, indicating an estimated $150 million contribution from ATTR cardiomyopathy. I will now provide some additional launch metrics to further contextualize Onwutra's launch performance in ATTR cardiomyopathy. Our launch began on March 20, 2025, and Q2 marked our first full quarter post-approval. It is still early, and there is more work to do, but we're very encouraged by the strong momentum we're seeing. As we've described on prior calls, we've been focused on three key enablers around the U.S. launch of Amutra in ATTR cardiomyopathy. Health system set up, access and affordability, and treatment choice. The headlines are here, and I will go into more detail on each in the following slides. As we've shared on prior calls, there are approximately 170 priority health systems through which approximately 80% of ATTR cardiomyopathy patient volume flows. The majority of these provider accounts now have Amutra on formulary, enabling therapy initiation throughout these health systems where ATTR-CM patients present. What's more, nearly all of the priority health systems have already begun treating patients with Amutra for ATTR cardiomyopathy. This, together with the broad network of more than 2,000 alternate sites of care, has allowed us to achieve our aspirations. roughly 90% of patients in the U.S. are able to receive armature treatment within about 10 miles of where they live. Bottom line, our priority was to enable broad provider account setup in our first year of launch. This has happened faster than we had initially anticipated. Since now, at the end of our first quarter of launch, we are largely there. In addition, patients are getting first-line access to Ambutra across all payer segments. Coverage is now confirmed by payers covering the majority of U.S. patient lives, inclusive of Medicare Fee-for-Service, Medicare Advantage, and commercial. We can therefore confirm that the large majority of patients have access to Ambutra as a first-line treatment, meaning without requiring patients to step through another product first. Most patients are indeed paying zero in out-of-pocket costs. And consistent with what we've seen in Poneuropathy, there has been very limited use of our Quick Start program, quite simply because patients are not experiencing delays in coverage. We're also seeing patient initiations flowing through all payer segments, Medicare Free-for-Service, Medicare Advantage, and commercial. These access dynamics are consistent with what we've long seen in HA-ATTR-PN, and we're encouraged to see them replicated in ATTR-CM. This reflects our deep experience engaging with payers and the advantage of our fully integrated in-house patient support services. Now, most importantly, physicians and patients are choosing Amutra, a testament to its highly differentiated, and compelling profile, including its rapid knockdown of the disease-causing protein. By the end of second quarter, approximately 1,400 ATTR-CM patients had initiated treatment. While we don't plan to regularly report patient numbers going forward, we felt it was important to share this clear signal of early momentum in our first full quarter post-launch. This strong uptake also gives us early insight into utilization patterns, which so far have been broad and balanced. More specifically, very early initial uptake was more pronounced among stabilizer progressors. However, within just three short months, utilization has become relatively balanced between first line, new starts, and stabilizer progressors. We're seeing steady growth across both sources of business. And we have a clear focus on making Amutra the first line treatment of choice. We also see balanced utilization across academic and community settings. And lastly, physician adoption has been broad. Since launch, the total Amutra prescriber base has tripled quarter over quarter. This reflects growing awareness and confidence in amateurs across both cardiology and multidisciplinary practices. Bottom line, we're highly encouraged by the early progress post-launch. The trajectory supports sustainable growth and positions us for long-term leadership in TTR amyloidosis. In summary, access ramped faster than expected, and the value proposition is resonating. We've seen rapid payer adoption and broad physician engagement. The clinical differentiator of Ambutra is clearly being recognized. We're seeing robust growth in an underserved and expanding ATTR-CM population. Look, this is a devastating disease, and we remain deeply committed to advancing care through real-world evidence generation and development of our next-generation RNAi therapeutics. Finally, global expansion is underway. With regulatory approvals secured in Europe, Japan, and Brazil, we've now launched in Germany and Japan, unlocking access to more patients worldwide. We're also maintaining stable growth in the HHTTR vulnerability business, both in the U.S. and globally. These drivers underpin our increased revenue guidance, and reinforce our conviction in significant revenue growth going forward. We're just getting started and we remain focused on discipline execution anchored in patient and customer centricity and delivering long-term innovation-driven growth. I'll now turn it over to Pushkal to share more about our work to advance the science in ATTR and beyond. Pushkal.

Thank you, Tolga, and good morning, everyone. I'm delighted to see the early success of Ambutra in these first few months of the launch. It is a true testament to the outstanding execution of our commercial and medical teams and to Ambutra's unique and compelling profile established in Helios B. To that end, we continue to generate evidence from the Helios B study that further supports the long-term efficacy and safety of Ambutra with the aim of cementing it as the first-line treatment of choice for patients with ATTR cardiomyopathy. This slide highlights some of the unique and profound benefits of Amvutra's rapid knockdown mechanism of action that are emerging from Helios B. In the left column, you can see the benefits on NT-proBNP and troponin I, important clinical biomarkers of cardiac stress and injury, respectively. Not only do you see a large effect in patients receiving drugs versus those on placebo, but it's interesting to see a reduction compared to baseline and troponin I. suggesting a potential disease-modifying effect on this biomarker. Further to that point, echocardiographic data in the middle column shows improvements in critical aspects of cardiac function, both diastolic and systolic. And ultimately, we saw this translate into substantial improvements in all-cause mortality, as well as cardiovascular mortality of 33% to 36%. In addition to data coming from the Helios B study, we are continuing to generate evidence to further support the safe and effective use of Ambutra by numerous registry and real-world evidence-based studies and investigator-initiated studies. We look forward to sharing data from these sources over time. Further to our leadership and commitment to innovation in ATTR amyloidosis, we announced in June the initiation of the Triton-CM Phase III study for Nucresiran, which may offer greater knockdown, greater efficacy, and greater convenience for patients with ATR cardiomyopathy. As a reminder, Triton-CM is a randomized, double-blind, event-driven outcome study. Patients are allowed to be on background stabilizer therapy. Approximately 1,200 patients will be randomized, and the primary endpoint is a composite of all-cause mortality and cardiovascular events. The primary analysis will be event-driven and will occur a minimum of 24 months after the last patient is enrolled. If successful, we target launching Nucresiran and ATTR cardiomyopathy around 2030. Also, As Yvonne noted earlier, we've announced today that Nucresiran has been granted fast-track designation by the FDA, which will enable a more streamlined review process. In parallel, we have the goal to bring Nucresiran to patients as quickly as possible and see an opportunity to do so in hereditary ATTR-PN. Though we are not yet prepared to discuss full details for Triton-PN, we remain on track to initiate a pivotal study for the polyneuropathy indication by the end of 2025. and anticipate the potential to launch in this indication several years ahead of cardiomyopathy. We look forward to sharing more details in due course. Moving on to another exciting program, just a few days ago, we presented new multi-dose data from the phase one study of mevelseran in patients with early onset Alzheimer's disease. Recall previously that we observed marked reductions in A-beta 40 and A-beta 42, the pathogenic proteins implicated in cerebral amyloid angiopathy and Alzheimer's disease, respectively, with single doses of mevelserine. We're pleased to see that continue with multiple doses administered every six months. In addition to the durable knockdown observed, the safety and tolerability profile also remains encouraging. The majority of AEs observed were non-serious, mild to moderate, and deemed unrelated to study drugs. Importantly, we saw no evidence of changes in CSF white blood cells, protein, or neurofilament-like change. which bodes well for this program, as well as our CNS platform. We look forward to further evaluating the profile of Mvelciran in the ongoing Capricorn phase two study in CAA, as well as the phase two study in Alzheimer's that we expect to start by the end of the year. As Yvonne mentioned earlier, a key element of our journey towards becoming a top tier biotech company is growth through innovation. Crossword is one of the most robust pipelines in the industry, we are making great progress advancing promising programs across a range of therapeutic areas with multiple potential blockbuster opportunities. In addition to some of the highlights we've already discussed, in Q2, we also kicked off a phase one trial for ALN4324 targeting GRV14 and insulin sensitizer for the treatment, potential treatment of type two diabetes. We also remain on track to start a phase two study of ALN6400 targeting plasminogen in a bleeding disorder later this year. In summary, Alnylam continues to make remarkable progress and deliver unique innovation that puts us in a great position to have a deep, sustainable pipeline that can deliver meaningful impact to patients for many years to come. With that, let me now turn it over to Jeff to review our financial results and upcoming milestones.

Jeff? Thanks, Pushkal, and good morning, everyone. I'm pleased to be presenting a summary of Alnylam's Q2 2025 financial results in discussing our full year upgraded guidance. Let's begin with a summary of our P&L results for the second quarter compared with prior year. Total product revenues for the quarter were 672 million or 64% growth versus 2024, driven by 77% growth in our TTR franchise, with particularly strong performance in the US market, primarily related to an increase in demand associated with the launch of InVutra and ATTR cardiomyopathy. Collaboration revenue for the quarter was $61 million, representing a $166 million decrease when compared with last year. The decrease was primarily driven by the modification of our Symdeciran collaboration agreement with Regeneron, which resulted in approximately $185 million of revenue in Q2 2024. As a reminder, this amendment granted Regeneron an exclusive license to Symdeciran monotherapy. Royalty revenue for the quarter was $40 million, representing an $18 million increase compared with last year, driven by higher Lectio sales. Gross margin on product sales was 79% for the quarter, compared with 84% in the second quarter of 2024. The decrease in margin was primarily driven by increased royalties Iambutra, as higher revenues in 2025 resulted in an increase in the royalty compared with last year. With the balance of the year, our gross margin on product sales is expected to decrease as the applicable Ambutra royalty rates increase, driven by higher sales of Ambutra. Our non-GAAP R&D expenses of $274 million increased 11%, primarily due to increases in startup clinical trial expenses associated with our cardiovascular outcomes trial for Zalvisaran and the Triton CM Phase III study for Nucresaran. Our non-GAAP SG&A expenses of $261 million increased 26% compared to last year, primarily driven by increased bed count and other investments in support of the MBUTRA ATTR cardiomyopathy launch in the U.S. Our non-GAAP operating income for the quarter was $95 million, representing a $42 million decrease compared with last year. driven primarily by the recognition of collaboration revenue related to the modification of our Regeneron agreement in Q2 2024, as I previously highlighted. We continue to be pleased with the progress we are making towards achieving our non-GAAP profitability guidance in 2025. Finally, we ended the quarter with cash equivalents and marketable securities of $2.9 billion compared to $2.7 billion as of December 31, 2024, with the increase primarily driven by cash from operations and that proceeds from the issuance of common stock in connection with employee stock option exercises. Now I'd like to turn to our financial guidance for 2025, where we are substantially increasing our net product revenue guidance, driven by the strong early launch performance of Ambutra and ATTR cardiomyopathy, with specific details as follows. We are increasing our net product revenue guidance from a range of 2.05 to 2.25 billion to a revised range of 2.65 to 2.8 billion, representing a 575 million or 27% increase from the midpoint of the prior guidance to the midpoint of the updated guidance. The combined full year growth compared to 2024 is a 66% increase at the midpoint of the guidance range. On a franchise level, the guidance is broken down as follows. We are modestly increasing the midpoint of our total rare franchise guidance by raising the bottom end of our prior guidance range by 25 million and leaving the top end of our guidance unchanged, resulting in revised rare product sales guidance of 475 to 525 million. We are materially increasing our total TTR guidance range from 1.6 to 1.725 billion to a revised range of 2.175 to 2.275 billion representing a 34% increase or more than 550 million at the midpoint. Let me provide some additional context, which highlights the impact of the cardiomyopathy launch on our TTR franchise growth based on our upgraded guidance. During 2024, when we only marketed our TTR therapies for HATTR polyneuropathy, our TTR franchise delivered approximately 300 million in revenue growth compared with 2023. In 2025, with the ATTR franchise now including cardiomyopathy sales following the US launch in Q2, the midpoint of our revised 2025 total TTR sales guidance of $2.23 billion represents an approximate $1 billion increase from 2024 total TTR sales of $1.2 billion. And now my last comment on our upgraded sales guidance. which now assumes foreign exchange rates as of June 30th for the balance of the year, resulting in approximately $60 million of our $575 million guidance increase being attributed to changes in FX from our original guidance, which utilized December 31st FX rates. The remainder of our financial guidance, including collaboration and royalty revenue, combined non-GAAP R&D and SG&A expenses, and non-GAAP operating income remains unchanged. Let me now turn from financials and discuss some key goals and upcoming 2025 milestones. In the second half of 2025, we expect to achieve the following. Initiate the Phase III CVOT of Zolbisorin and Hypertension. Note that we also announced today that we'll present Cardio III Phase II results at the European Society of Cardiology Congress later this summer. Initiate the Triton-PN Phase III study of Nucrecerin and HATTR-PN and initiate Phase II studies for myvelseran and Alzheimer's disease and AL6400 in a bleeding disorder. Let me now turn it back to Christine to coordinate our Q&A session. Christine?

Thank you, Jeff. Operator, we will now open the call for questions. To those dialed in, we would like to ask you to limit yourself to one question each and then get back in the queue if you have additional questions.

Thank you. Ladies and gentlemen, we will now begin the question and answer session. Should you have a question, please press the star followed by the number one on your touchtone phone. You will hear a prompt that your hand has been raised. Should you wish to decline from the polling process, please press the star followed by the number two. If you are using a speakerphone, please leave the handset before pressing any keys. One moment please for your first question. Your first question comes from Richter Salvi with Goldman Sachs. Please go ahead.

Good morning. Thanks for taking my question, and congratulations here on the quarter. Could you give some details here in the patient profiles for MVUTRA with regard to the frontline patients and whether you're seeing mostly mixed phenotype patients or more of a broader population mix? And help us understand from here what the field force is most focused on. Thank you.

Thanks, Saville. I mean, I'll start by saying, look, you know, it's early days yet, but we're incredibly pleased with where the launch is going, particularly with, you know, broad and robust uptake. Because I think it also really shows that the data that we generated from HDSB is really resonating, you know, with physicians. You know, the rapid knockdown of TTR, all-cause mortality improvements, infrequent subcutaneous administration, which provides both convenience and security, So, you know, we're seeing broad uptake across, you know, first-line patients as well as patients that progress on stabilizers. We're seeing use across the community physicians as well as academic physicians, as well as new prescribers, as well as repeat prescribers. So the trends are really very supportive in suggesting continued growth. Now, I think it's fair to say that the initial pickup has been faster in the stabilizer-progressing patients because you bring in a new therapy and, you know, the patients who've been progressing on stabilizers, it's not surprising that, you know, physicians are looking for an orthogonal treatment for these patients. But we're now achieving a really healthy share of first-line patients. So we have a good mix of first-line stabilizer progressors. And what we're going to be doing is really consolidating and building on the foundations of this very early picture. Tolga, you probably want to add a couple of comments.

Actually, you pretty much covered everything. What I would say is, again, we're just one quarter in, and what we're already seeing, what you described, is validates our clinical thesis and commercial strategy.

Thanks, Tolga. Next question.

Thank you. The next question comes from Tazeen Ahmad with Bank of America. Please go ahead.

Hi, good morning. Thanks for taking my question. I just wanted to get a sense on how you're thinking about net price for Ambutra and gross to net moving forward.

Yeah, no, thanks for that question. Jeff, perhaps you'd like to start off answering that, and if there are any additional perspectives, Tolga and I can follow on from you.

Yeah, just a reminder of what we said back on the approval call in March. You know, we said that we expected that we would reduce net price modestly and gradually over time, and that does hold for our expectation for 2025. 2025 relative to 2024, I would expect mid-single-digit reduction in net price for Ambutra for the year.

Okay. Yeah, I mean, look, I think one thing is also to importantly highlight is the fact that payer dynamics are moving very much as what we exactly expected. A lot of the payer policies are already out, both on Medicare Advantage as well as in commercial. And what we're seeing is a first-line access to those patients and patients actually paying minimal, in most cases, zero out-of-pocket costs. So we'll obviously manage that with early engagement with payers. And the impact of the gross net is going to be, as Jeff highlighted, will evolve over time.

Thanks. Thanks, Jeff and Tolga.

Next question. Thank you. The next question comes from Marie Raycroft with Jefferies. Please go ahead.

Hi, good morning, and congrats on the great quarter, and thank you for taking our question. I'm wondering if you can comment on whether there was any bolus effect in cardiomyopathy scripts in second quarter, and could you also please clarify to what extent did stocking contribute to the growth of U.S. on blue-tribe revenue in the second quarter?

That's a great question. Look, you know, I think Tolga's touched on this. You know, we've really demonstrated very solid commercial execution And that means that we've seen a little bit of faster progress than we expected, particularly around health system setup. So I touched on this already, but it's meant that we had initial faster pickup with stabilizer progresses. But as we went through the queue, we now have this very healthy share of first line. And we're just in the first quarter of launch. I think the key message, number one, for me, is that the results that we're sharing with you today are not just a flash in the pan. We expect continued sustainable growth, and that's the reason why we raised guidance today. And I think the second key message is really just the durability of the franchise. We expect to see Ambutra deliver continued sustainable growth, but we'll be bringing Nucreceram forward as well, as Prishkal highlighted, which gives us the potential to deliver sustainable franchise growth out into the 2040s?

I'll comment on the second part of the question, which was about inventory. I think the headline for the quarter is, again, in the U.S., Tolga and his prepared remarks highlighted 170 million in growth in the TTR franchise in the U.S., Q2 versus Q1. Far and away, the biggest driver of that was the cardiomyopathy demand. 1,400 patients on therapy, $150 million in cardiomyopathy revenue for the quarter is what we're estimating. On the inventory side, we did see about a $25 million benefit in Q2 compared to Q1. That's not actually because days on hand increased between Q1 and Q2, as it actually stayed constant at around 20 days, which is at about the midpoint of the agreements that we've got in place with our distributors. What drove the increase in inventory in the quarter was the calculation of what a day of demand is, or a day of inventory is worth, which is based on a 12-week moving average of demand. And so it was demand... that really drove the increase in inventory and the channel in the quarter, which is, again, a high-quality increase in channel stocking in the quarter. Now, on the gross-to-net side, we had an opposite movement, so a headwind that roughly offset the benefit that we had in inventory, which is why we're highlighting demand was the key driver of growth in the quarter. On the gross-to-net side, a couple things. One is that We had an increase associated with a Part D rebate that we're paying. Anybody that's got a Part D drug at this point is paying rebates on the IRA Medicare redesign. We do have a small portion of our TTR sales that go through the Part D channel, and that's the home care part of the business. Historically, that's been about 20%. and polyneuropathy. So there is an increased rebate that we're paying there, which was in line with our expectations. We also saw modestly higher 340B utilization in the quarter. So on price, net price, Q2 to Q1 was down slightly, which is consistent with what I said on the earlier question, that we do expect net price 25 versus 24 for Ambutra to be down mid-single digits.

Thanks, Jeff. I think a very important question, and I think you've clarified that for are invested. So thank you. Next question, please.

Thank you. The next question comes from Jessica Fy with JP Morgan. Please go ahead.

Hey, guys. Good morning. Thanks for taking our question. Can you speak a little more in a little more detail to the assumptions underpinning the updated TTR franchise guidance as it relates to like the pace of new starts And I guess just in general, can you just provide a little assurance or reaffirm that the new guidance is not somehow aggressive? Thank you.

Okay, so two parts to that question. Maybe, Tolga, if you start with the first part, and then Jeff, you can follow on from that.

Yeah, absolutely. Hi, Jessica. I mean, look, at the end of the day, what we've already laid out is we're expecting a steady growth in both first-line as well as the second line patient flow. As Yvonne indicated, let me also provide a little additional color. As expected, we initially saw uptake concentrated patients who had progressed on stabilizers. And these are often sicker patients. Physicians naturally look for an alternative therapy with a mechanism of action that's unique, that's accessible, easy to administer, and so forth. But what's been really particularly encouraging, and this is why the guidance we're providing is over half a billion dollar optic from where we were, is about a month into the quarter, we began seeing a very healthy and accelerating trend in first-line use, just as we had positioned from the outset. And frankly, that makes a lot of sense. This is a progressive and fatal disease. Physicians want to hit the disease early, and it is hard. and not hold back their best therapy for later, as they might in less serious conditions. So today, and this is where the guidance is really anchored in, we see both first-line and second-line segments are growing. They're growing rapidly. Uptake is broad across first and second line and academic and community. And more importantly, we're seeing a good expansion of our prescriber base. We've just expanded by threefold, and that continues to move in the right direction. So as we move forward, our focus is clear. To continue reinforcing Amutra as the first-line treatment of choice, supported not only by our approved label, but also by growing evidence, as Pushkal highlighted, with new imaging data, emerging cardiac biomarker trends that suggest Amutra may even help reverse disease progression. So nonetheless, we're just one quarter in. and already seeing that behavior that validates our clinical profile as well as our commercial ambitions. And I think the current guidance really reflect that.

Yeah, look, I think I just want to emphasize that it's early days yet. We've only just achieved our first full quarter here. And when we came up with the guidance, we've given guidance that we expect to achieve. We're going to be focusing our team on continued execution. And as we progress, we will, you know, continue to update and share our perspectives.

Yeah, maybe just a little bit more context on the guidance. Again, if you look at the, you know, what I highlighted in terms of the midpoint of the guidance and what it implies in terms of year-over-year growth for TTR, about a billion dollars, right, is what I highlighted. Last year, the PN franchise grew 300 million versus 23 and was growing at about 30%, right? So if you just sort of assume that that's the growth that we're going to get from PM this year, that would imply 600 million plus from cardiomyopathy. We just did 150 million in the second quarter, and we feel really good about growth sustaining through the second half of the year. So I do think we have a high level of confidence in the guidance that we provided, Jess.

Thanks, Jeff.

Next question. Thank you. The next question comes from Paul Mattis with Stifel. Please go ahead.

Hey, great. Thanks so much for taking my question. One for Tolga and the team. As it relates to stabilizer progressors, can you talk a little bit more about the criteria physicians are using? And I guess now that you've been in the market, do you have a sense of what percent of patients who are on a stabilizer currently would be dictated to be a progressor and a good candidate for Mvutra based on the clinical criteria that are being implemented? Thanks so much.

Yeah, so we'll start with Tolga and then I'd like Pushkar to provide a clinical perspective.

Yeah, so Paul, I mean, look, based on our research and obviously published data, clinical evidence suggests that anywhere between third to half the patients on stabilizer at one point progresses. And what we see that number is continuing to grow. And then obviously the only real option in terms of mechanistically and the clinical evidence suggests that, you know, it would be Ambutra at this point. But let me turn it over to Pushkal, who can also provide some more, you know, clinical perspective about what we're seeing and how those guidelines are actually progressing. Yeah, thanks, Tolga.

Thanks, Paul. Look, I think it's really important. This is a, you know, we talk about ATTR cardiomyopathy, and the thing here is that it has a clear cause, which is accumulation of TTR protein in the, you know, But as it clinically presents, it's a form of heart failure. And doctors have been treating heart failure for decades, and they know how to modify drug therapy for patients. They might add diuretics or SGLT2s or other agents as patients are progressing. And so I think they're well accustomed to actually looking at a variety of factors. And frankly, you have to rely on a variety of factors. There's not any one specific indicator. An easy way to think about that is when you look at the European guidelines that were put out a couple of years ago, and they had a variety of considerations, including biomarkers, echocardiographic factors, exercise tolerance, patients' ability to lie flat at night, different things, pedal edema. And so I think what we're seeing now in these early days is that doctors are applying that general clinical rubric of looking at a patient, how they present, their symptoms, their signs, and other factors in terms of determining how patients will progress. We do think that over time, more and more guidelines in this category will develop over time, but our expectation is that there will not be some one single factor that clinicians or payers will be able to rely upon to say specifically that a patient's progressing, but we'll rather have to look at the constellation of factors that affect the patient's symptomatology.

Thanks, Pascal.

Thank you.

We'll take the next question. Thank you. The next question comes from Luca Izzi. with RBC Capital. Please go ahead.

Oh, great. Thanks so much for taking my question. Congrats on the strong quarter. Maybe if I can circle back on the TTR guide, maybe Jeff, like if I assume $200 million in revenue for the year from PATRO, that essentially means $550 to $650 million in revenues for the next two quarter for Ambutra, which is actually not dissimilar from the $492 million that you already printed today. I guess what I'm trying to say feels to me that this guy has still a good degree of conservatism in it, especially given that you're going to start selling this drug in international markets like Brazil, Europe, UK, Japan, etc., But we'd love if you have a different view here. So any thoughts there, much appreciated. Thanks so much.

Yeah, Luca, I appreciate the question. I'll again reiterate what I said to the earlier question here. We're guiding to a billion dollars of TTR growth year over year. Last year, PN grew 300, and it's growing at about 30%. So that gets you to 600 plus for cardiomyopathy. We just did 150 million in Q2. I think we feel very confident about that. If you look at historically the guidance that we've given and the consistency with which we either met that or exceeded that, that's how we feel about the guidance that we're providing. I don't know if it's conservative at this point. Honestly, we're one quarter into this, and certainly we look forward to coming back at Q3 and reassessing things.

Thank you. Thanks so much.

Next question. Thank you. The next question comes from Ellie Merle with UBS. Thank you.

Hey, guys. Congratulations on the quarter, and thanks so much for taking the question. Just to drill into this a little bit more, how should we think about the rate of new patient starts per quarter from here? 1,400 is obviously a phenomenal number. Should we think of this cadence of new starts continuing in 3Q and beyond? And second, in terms of the mix, You mentioned now seeing more balanced starts mix between the newly diagnosed and the progressors, whereas initially it was more of the progressors. How do you expect this mix to evolve over time from here? Thank you.

Okay, so I think two questions. You can probably take both of them, Salga.

Yeah, so thank you. Look, at the end of the day, we're very pleased, obviously, with 1,400 paid patients within a three-month period. And as you had highlighted, while the patient starts initially was more predominantly stabilizer progresses very quickly, that switched into a balanced and broad patient uptake. We certainly expect to see both of those categories continue to grow. Now, in terms of the specific numbers, we kind of went out of our way to be able to provide that clarity. As you know, Ambutra has a single SKU, so we can't really isolate CM patients with precision. So we're going to continue to provide additional color, and those numbers obviously we expect to go up. But in terms of the specific precision about how it's going to go quarter after quarter is not going to be, we're not going to be able to report that.

Thank you. Next question. Thank you. The next question comes from Costas Boullieris with BMO Capital Market. Please go ahead.

Thanks for taking our question and congrats on the impressive launch. One question on payers from us, although you already touched a little bit on that. We have seen some commercial payers requiring stabilizer use prior to Amvutra treatment in cardiomyopathy. Can you comment on how common those requirements are across the different plans and what percentage of patients do these plans cover? Thank you.

Yeah, I know that's a great question. I mean, I'll just start off by saying that as we look at this, you know, access is just not a barrier. We're seeing broad coverage across, you know, Medicare, fee-for-service, Medicare Advantage, commercial payers, and the vast majority of patients are getting first line with no step. And this is exactly what we predicted coming into the launch and what we've been working on for quite some time. I think it really speaks to the Ambuta profile as well as the nature of the disease. But Tolbert, you might want to add another perspective.

You got it, Yvonne. I think the broad headline is really, as predicted, we're not facing a significant headwind in terms of payer coverage. What I'm really pleased to see was within a short three-month period, majority of both Medicare Advantage as well as commercial payers, have published policies. And in those policies, Almutra, in broad strokes, are covered first line. Now, we also had actually flagged that there could be some commercial payers that could actually provide a SEP edit in their policies. We're seeing that, but it is incredibly minimal. It's in the single digits and frankly, we don't anticipate that to continue to grow as most other large commercial payers have already written policies that cover Zambutra's first line. Now, when it comes to step edits, we have the tools and the support systems that enables us to actually, frankly, to help patients and providers to circumvent that or make sure that it's managed very, very carefully. This is a We've actually built over the years a quiet engine, driving real impact in terms of our patient services. And I'm really pleased to see how we've been able to pull through on all three segments. Patients are already getting on treatment, whether it's fee-for-service, whether it's Medicare Advantage. also on commercial payers, including those plans that have actually separated. So we don't see that for now, and obviously we're going to close the monitor and continue to engage with payers to make sure that these patients that deal with the severe condition are getting a seamless access with our medicines.

Yeah, that looks great. And one data point that really struck me was actually just the minimal use we're seeing of our Quick Start program. we introduced a quick start program with all of our launches to make sure that we can help patients with access. And we're just not seeing much use, which I think, again, is very encouraging. Next question, please.

Thank you. The next question comes from Gina Wang with Barclays. Please go ahead.

Thank you for taking my questions. I also wanted to congrats on the outstanding quarter. So maybe Yvonne, I think you mentioned that the vast majority will be the first-line patient. Is it fair to say that out of 1,400 patients treated so far, it would be over 50% of patients is a first-line patient? And then out of this 1,400 patients, how many of them receive free drug?

Bobo, you love breaking this down.

Yeah. Look, Gina, good to hear from you. I think what we had said very thoughtfully, I would say, is our update has been broad and balanced. So that included, again, early on, some patients that were actually, you know, progressing on stabilizers. And then what we're seeing, a very, very strong trend of first-line indication. We're in the early innings. And I think the job is to, and we're getting, I would say, our fair share of first-line patients, but the job is not done yet. What we want to make sure is that we continue to this trend and make sure that we continue to educate both patients as well as prescribers why Ambutra has a compelling product profile to be a first-line patient. So I just wanted to reiterate that. Yeah, it's really important that we...

I think she also had a question about the 1,400 patients and how many of them were on the Quick Start program. It was de minimis, right? It was very, very low. It was absolutely very minimal.

Next question. Thank you. The next question comes from Mike Ulf with Morgan Stanley. Please go ahead.

Good morning. Thanks for taking the question, and congrats on the strong launch as well. Maybe just to follow up on TTR cardiomyopathy, you highlighted you're getting some nice broad use in the front line as well as the stabilizer-progressor patients. Just curious if you started to see any combination use early in the launch. I know you're not expecting it, but we've picked up some combination use in some of our KOL calls.

Yeah, no, thank you. Thank you for that question. Toga, why don't you take that?

Yeah, look, we certainly do see a very small portion of those patients getting a combination use. We would certainly expect that as tefamidus goes generic over the years to become more prominent. But right now, it's really difficult to be very specific about looking at the specific combination use. But I think in where it's allowed and where access is permissible, We do some utilization of Combo.

Thank you. Thank you. I think we've got time for one last question.

Yes. The next question comes from Ritsu Baral with T.D. Cohen. Please go ahead.

Hi, guys. Thanks for squeezing me in, and congratulations on the quarter. question on variants. You guys might address the mixed phenotype, but in your first line and first line access, what trends are you seeing in the V122i variant population? As KOL feedback, at least on my model, has suggested, I'm underestimating that prevalence by about 6x, but it is a more severe prevalence. phenotypic presentation and just a very quick follow-up. This is probably semantics, but you guys, Tolga, you've mentioned step through and step edits. Are they the same thing as prior authorizations or do you have a different set of prior authorizations? Thanks.

Yeah, so let me take your last part first. The step edit policies that we've seen, again, it's incredibly small. It's in the single digit and it's mostly predominantly in the commercial setting. There are no real specific limitations other than patients putting on a stabilizer first. And frankly, there's not even a time limit or duration. And it's really up to the physician if the patient is progressing. So we find these policies relatively easy to manage for the patients. And there are no specific hereditary or V122Is. And frankly, we're not surprised about that. We have, I think, what we've demonstrated early on with the pulmonary hereditary condition, and then later with the broad cardiomyopathy label, is that Ombutzra is well-positioned to be a first-line patient for all diagnosed ATTR-CM patients. And we're really not seeing that trend. Maybe I'll turn it over to Pushkal if he has any specific commentary on the trial.

I think you said it well. Look, I think, you know, what we've seen in Helios B is that Ambutra works equally well in wild-type patients and in V122I patients. And as Tolga mentioned, this is the one class of drugs that's shown actually benefit in hereditary patients, which constitutes the wide range of mutations, and we've seen benefits across that. So across, I think, all ranges of severity, whether it's NYHA class 123, whether it's hereditary or wild type, I think, you know, that's, I think, what really cements our belief that this is really, and what we're hearing from prescribers in terms of the opportunity to treat patients with this drug as a first-line agent, Ritu.

Thank you, Ritu. I think this brings our call to a close, and I'd just like to, you know, thank everybody for joining us today on this call. Look, our line must continue to execute strongly across all areas of the business, and we're very much looking forward to providing you further updates as we progress throughout the year. Thanks a lot.

Ladies and gentlemen, this concludes today's conference call. Thank you for your participation. You may now disconnect.